human sperm head morphology dataset Search Results


human sperm dna  (New England Biolabs)
96
New England Biolabs human sperm dna
Human Sperm Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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herring sperm dna  (Thermo Fisher)
99
Thermo Fisher herring sperm dna
Herring Sperm Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spermatozoa  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology spermatozoa
Spermatozoa, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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spag6  (Proteintech)
93
Proteintech spag6
Fig. 5. Effect of MC-LR exposure on human sperm flagellum A. The “9 + 2′′ structure of the sperm flagellum was observed using transmission electron microscopy. Red arrows: the CMs was asymmetrically distributed. Green arrows: the MDs were off to one side. Yellow arrows: the MDs were absent. B. The mRNA levels of SPAG16, <t>SPAG6,</t> CFAP44, DNAH17, and SPEF2 were measured by RT-qPCR. C and D. The location of DNAH17 and SPEF2 proteins in sperm were assessed using Immunofluorescence. E and F: The expression levels of sperm flagellum-associated proteins SPAG16, <t>SPAG6,</t> and CFAP44 were detected using Western blotting. The internal reference protein is GAPDH. All exposure times were chosen to be 6 h. * represented statistically significant differences (p < 0.05) compared to the control group in the same period.
Spag6, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human sperm functions  (Schill Seilacher GmbH)
90
Schill Seilacher GmbH human sperm functions
Fig. 5. Effect of MC-LR exposure on human sperm flagellum A. The “9 + 2′′ structure of the sperm flagellum was observed using transmission electron microscopy. Red arrows: the CMs was asymmetrically distributed. Green arrows: the MDs were off to one side. Yellow arrows: the MDs were absent. B. The mRNA levels of SPAG16, <t>SPAG6,</t> CFAP44, DNAH17, and SPEF2 were measured by RT-qPCR. C and D. The location of DNAH17 and SPEF2 proteins in sperm were assessed using Immunofluorescence. E and F: The expression levels of sperm flagellum-associated proteins SPAG16, <t>SPAG6,</t> and CFAP44 were detected using Western blotting. The internal reference protein is GAPDH. All exposure times were chosen to be 6 h. * represented statistically significant differences (p < 0.05) compared to the control group in the same period.
Human Sperm Functions, supplied by Schill Seilacher GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d systems r2000 chromatin immunoprecipitation chip antibodies histone h3k9me3  (R&D Systems)
93
R&D Systems d systems r2000 chromatin immunoprecipitation chip antibodies histone h3k9me3
Fig. 5. Effect of MC-LR exposure on human sperm flagellum A. The “9 + 2′′ structure of the sperm flagellum was observed using transmission electron microscopy. Red arrows: the CMs was asymmetrically distributed. Green arrows: the MDs were off to one side. Yellow arrows: the MDs were absent. B. The mRNA levels of SPAG16, <t>SPAG6,</t> CFAP44, DNAH17, and SPEF2 were measured by RT-qPCR. C and D. The location of DNAH17 and SPEF2 proteins in sperm were assessed using Immunofluorescence. E and F: The expression levels of sperm flagellum-associated proteins SPAG16, <t>SPAG6,</t> and CFAP44 were detected using Western blotting. The internal reference protein is GAPDH. All exposure times were chosen to be 6 h. * represented statistically significant differences (p < 0.05) compared to the control group in the same period.
D Systems R2000 Chromatin Immunoprecipitation Chip Antibodies Histone H3k9me3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prehybridisation solution  (Thermo Fisher)
99
Thermo Fisher prehybridisation solution
Fig. 5. Effect of MC-LR exposure on human sperm flagellum A. The “9 + 2′′ structure of the sperm flagellum was observed using transmission electron microscopy. Red arrows: the CMs was asymmetrically distributed. Green arrows: the MDs were off to one side. Yellow arrows: the MDs were absent. B. The mRNA levels of SPAG16, <t>SPAG6,</t> CFAP44, DNAH17, and SPEF2 were measured by RT-qPCR. C and D. The location of DNAH17 and SPEF2 proteins in sperm were assessed using Immunofluorescence. E and F: The expression levels of sperm flagellum-associated proteins SPAG16, <t>SPAG6,</t> and CFAP44 were detected using Western blotting. The internal reference protein is GAPDH. All exposure times were chosen to be 6 h. * represented statistically significant differences (p < 0.05) compared to the control group in the same period.
Prehybridisation Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pre hybridization buffer  (Thermo Fisher)
99
Thermo Fisher pre hybridization buffer
Fig. 5. Effect of MC-LR exposure on human sperm flagellum A. The “9 + 2′′ structure of the sperm flagellum was observed using transmission electron microscopy. Red arrows: the CMs was asymmetrically distributed. Green arrows: the MDs were off to one side. Yellow arrows: the MDs were absent. B. The mRNA levels of SPAG16, <t>SPAG6,</t> CFAP44, DNAH17, and SPEF2 were measured by RT-qPCR. C and D. The location of DNAH17 and SPEF2 proteins in sperm were assessed using Immunofluorescence. E and F: The expression levels of sperm flagellum-associated proteins SPAG16, <t>SPAG6,</t> and CFAP44 were detected using Western blotting. The internal reference protein is GAPDH. All exposure times were chosen to be 6 h. * represented statistically significant differences (p < 0.05) compared to the control group in the same period.
Pre Hybridization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti protamine 2 prm2 antibody  (Proteintech)
91
Proteintech anti protamine 2 prm2 antibody
A The Kmal in total proteins of (TP) and protein fractions of the mitochondrion (Mit), head, and cytoplasm (Cyt) isolated from human sperm were examined by Western blot. The specificity of cellular components was validated by Western blot using the corresponding markers: <t>PRM2</t> for sperm head, COX6B1 for mitochondrion, and ACTIN for cytoplasm. The proteins for Western blot were shown in the coomassie brilliant blue (CBB) staining image. B , C The localization of malonylated proteins within human sperm was investigated by immunofluorescence assay via laser scanning confocal microscopy (LSCM, ( B )) and super-resolution structured illumination microscopy (SIM, ( C )). All the experiments were conducted with samples from 8 normozoospermic men. The scale bar represents 5 μm.
Anti Protamine 2 Prm2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti zp3  (Proteintech)
94
Proteintech anti zp3
A The Kmal in total proteins of (TP) and protein fractions of the mitochondrion (Mit), head, and cytoplasm (Cyt) isolated from human sperm were examined by Western blot. The specificity of cellular components was validated by Western blot using the corresponding markers: <t>PRM2</t> for sperm head, COX6B1 for mitochondrion, and ACTIN for cytoplasm. The proteins for Western blot were shown in the coomassie brilliant blue (CBB) staining image. B , C The localization of malonylated proteins within human sperm was investigated by immunofluorescence assay via laser scanning confocal microscopy (LSCM, ( B )) and super-resolution structured illumination microscopy (SIM, ( C )). All the experiments were conducted with samples from 8 normozoospermic men. The scale bar represents 5 μm.
Anti Zp3, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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shaker  (Proteintech)
93
Proteintech shaker
A The Kmal in total proteins of (TP) and protein fractions of the mitochondrion (Mit), head, and cytoplasm (Cyt) isolated from human sperm were examined by Western blot. The specificity of cellular components was validated by Western blot using the corresponding markers: <t>PRM2</t> for sperm head, COX6B1 for mitochondrion, and ACTIN for cytoplasm. The proteins for Western blot were shown in the coomassie brilliant blue (CBB) staining image. B , C The localization of malonylated proteins within human sperm was investigated by immunofluorescence assay via laser scanning confocal microscopy (LSCM, ( B )) and super-resolution structured illumination microscopy (SIM, ( C )). All the experiments were conducted with samples from 8 normozoospermic men. The scale bar represents 5 μm.
Shaker, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fibrinogen  (Angio-Proteomie)
86
Angio-Proteomie fibrinogen
A The Kmal in total proteins of (TP) and protein fractions of the mitochondrion (Mit), head, and cytoplasm (Cyt) isolated from human sperm were examined by Western blot. The specificity of cellular components was validated by Western blot using the corresponding markers: <t>PRM2</t> for sperm head, COX6B1 for mitochondrion, and ACTIN for cytoplasm. The proteins for Western blot were shown in the coomassie brilliant blue (CBB) staining image. B , C The localization of malonylated proteins within human sperm was investigated by immunofluorescence assay via laser scanning confocal microscopy (LSCM, ( B )) and super-resolution structured illumination microscopy (SIM, ( C )). All the experiments were conducted with samples from 8 normozoospermic men. The scale bar represents 5 μm.
Fibrinogen, supplied by Angio-Proteomie, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 5. Effect of MC-LR exposure on human sperm flagellum A. The “9 + 2′′ structure of the sperm flagellum was observed using transmission electron microscopy. Red arrows: the CMs was asymmetrically distributed. Green arrows: the MDs were off to one side. Yellow arrows: the MDs were absent. B. The mRNA levels of SPAG16, SPAG6, CFAP44, DNAH17, and SPEF2 were measured by RT-qPCR. C and D. The location of DNAH17 and SPEF2 proteins in sperm were assessed using Immunofluorescence. E and F: The expression levels of sperm flagellum-associated proteins SPAG16, SPAG6, and CFAP44 were detected using Western blotting. The internal reference protein is GAPDH. All exposure times were chosen to be 6 h. * represented statistically significant differences (p < 0.05) compared to the control group in the same period.

Journal: Ecotoxicology and environmental safety

Article Title: Microcystin leucine arginine induces human sperm damage: Involvement of the Ca 2+ /CaMKKβ/AMPK pathway.

doi: 10.1016/j.ecoenv.2023.114845

Figure Lengend Snippet: Fig. 5. Effect of MC-LR exposure on human sperm flagellum A. The “9 + 2′′ structure of the sperm flagellum was observed using transmission electron microscopy. Red arrows: the CMs was asymmetrically distributed. Green arrows: the MDs were off to one side. Yellow arrows: the MDs were absent. B. The mRNA levels of SPAG16, SPAG6, CFAP44, DNAH17, and SPEF2 were measured by RT-qPCR. C and D. The location of DNAH17 and SPEF2 proteins in sperm were assessed using Immunofluorescence. E and F: The expression levels of sperm flagellum-associated proteins SPAG16, SPAG6, and CFAP44 were detected using Western blotting. The internal reference protein is GAPDH. All exposure times were chosen to be 6 h. * represented statistically significant differences (p < 0.05) compared to the control group in the same period.

Article Snippet: The antibodies of SPAG16, SPAG6, DNAH17, and GAPDH were obtained from Proteintech Co., ltd (Wuhan, China).

Techniques: Transmission Assay, Electron Microscopy, Quantitative RT-PCR, Immunofluorescence, Expressing, Western Blot, Control

Fig. 7. Possible mechanism of MC-LR-caused sperm dysfunction and flagellar structural damage through the Ca2þ/CaMKKβ/AMPK pathway. After entering human sperm, MC-LR inhibits the phosphorylation of CaMKK by reducing the concentration of calcium ions in the sperm, further resulting in a decrease in the phosphorylation of AMPK. The inhibition of the Ca2+/CaMKKβ/ AMPK pathway reduces flagellar structural proteins (CFAP44, DNAH17, SPEG2 and SPAG6/16) and decreases viability, motility and capacitation of human sperm.

Journal: Ecotoxicology and environmental safety

Article Title: Microcystin leucine arginine induces human sperm damage: Involvement of the Ca 2+ /CaMKKβ/AMPK pathway.

doi: 10.1016/j.ecoenv.2023.114845

Figure Lengend Snippet: Fig. 7. Possible mechanism of MC-LR-caused sperm dysfunction and flagellar structural damage through the Ca2þ/CaMKKβ/AMPK pathway. After entering human sperm, MC-LR inhibits the phosphorylation of CaMKK by reducing the concentration of calcium ions in the sperm, further resulting in a decrease in the phosphorylation of AMPK. The inhibition of the Ca2+/CaMKKβ/ AMPK pathway reduces flagellar structural proteins (CFAP44, DNAH17, SPEG2 and SPAG6/16) and decreases viability, motility and capacitation of human sperm.

Article Snippet: The antibodies of SPAG16, SPAG6, DNAH17, and GAPDH were obtained from Proteintech Co., ltd (Wuhan, China).

Techniques: Phospho-proteomics, Concentration Assay, Inhibition

A The Kmal in total proteins of (TP) and protein fractions of the mitochondrion (Mit), head, and cytoplasm (Cyt) isolated from human sperm were examined by Western blot. The specificity of cellular components was validated by Western blot using the corresponding markers: PRM2 for sperm head, COX6B1 for mitochondrion, and ACTIN for cytoplasm. The proteins for Western blot were shown in the coomassie brilliant blue (CBB) staining image. B , C The localization of malonylated proteins within human sperm was investigated by immunofluorescence assay via laser scanning confocal microscopy (LSCM, ( B )) and super-resolution structured illumination microscopy (SIM, ( C )). All the experiments were conducted with samples from 8 normozoospermic men. The scale bar represents 5 μm.

Journal: Communications Biology

Article Title: Lysine malonylation regulates human sperm motility

doi: 10.1038/s42003-026-09683-y

Figure Lengend Snippet: A The Kmal in total proteins of (TP) and protein fractions of the mitochondrion (Mit), head, and cytoplasm (Cyt) isolated from human sperm were examined by Western blot. The specificity of cellular components was validated by Western blot using the corresponding markers: PRM2 for sperm head, COX6B1 for mitochondrion, and ACTIN for cytoplasm. The proteins for Western blot were shown in the coomassie brilliant blue (CBB) staining image. B , C The localization of malonylated proteins within human sperm was investigated by immunofluorescence assay via laser scanning confocal microscopy (LSCM, ( B )) and super-resolution structured illumination microscopy (SIM, ( C )). All the experiments were conducted with samples from 8 normozoospermic men. The scale bar represents 5 μm.

Article Snippet: Anti-ACTIN antibody (66009-1-Ig), anti-SIRT5 antibody (67257-1-Ig), anti-acetyl-CoA carboxylase 1 (ACC1) antibody (21923-1-AP), anti-fatty acid synthase (FASN) antibody (10624-2-AP), anti-protamine 2 (PRM2) antibody (14500-1-AP), anti-GAPDHS (83290-3-RR) and anti-VDAC3 (82666-14-RR) were obtained from Proteintech Group, Inc. (Rosemont, IL, USA).

Techniques: Isolation, Western Blot, Staining, Immunofluorescence, Confocal Microscopy, Microscopy